fam dye wavelength

trailer 0000049106 00000 n The ABY® and JUN® reporter dyes complement our existing offering of FAM â¢ and VIC® reporter dyes for multiplexing. 0000011716 00000 n 0000042037 00000 n 0 It is widely used as a fluorescent tracer for many applications. 0000000896 00000 n It can be excited using a 640 nm laser paired with a 670/30 nm bandpass filter, a configuration that can be found, for example, in the BD FACSAriaâ¢ Fusion. 0000005860 00000 n Many derivatives of fluorescein are used in research, including FITC, FAM, and carboxyfluorescein. Background FAM, JOE, TAMRA and ROX, the "big four" dyes, have dominated DNA sequencing. 0000014285 00000 n The principles of fluorescence and the synthesis of fluorescent oligonucleotides are described below. 0000017395 00000 n 6-FAMâ¢ is the most commonly used fluorescent dye for attachment to oligonucleotides and is compatible with most fluorescence detection equipm ent. 0000011845 00000 n 0000034128 00000 n 0000001717 00000 n In addition alternative laser lines are becoming more affordable so dyes excited by 355 nm and 405 nm lasers are increasing the options for multiplexing. This suggests successful detection of active TdT using FAM and Cy5 FRET pairs. 0000026076 00000 n 0000045848 00000 n 0000051527 00000 n It is available as a dark orange/red powder slightly soluble in water and alcohol. 0000051199 00000 n 0000021999 00000 n 500. Following absorption, ï¬uorescence emission may be of central importance as occurs in various ï¬uorometric analytical procedures (e.g. 0000048744 00000 n 0000026489 00000 n Single dyes such as FITC, PE, APC and PerCP have been available for many years, but there are now alternatives available from Alexa Fluor dyes, which offer users greater photostability and brighter fluorescence. The major applications of fluorescent and fluorogenic oligonucleâ¦ In the US, call 1-800-4BIORAD (1-800-424-6723). A red wavelength dye commonly used in multiplex reactions with FAM or in fluorescence-activated cell sorting (FACS) and microscopy applications using the rhodamine filter. 0000042297 00000 n FAM Emission. 0000010927 00000 n It provides better solubility, and spatial separation between the dye, and the functional group. Table 1 provides a list of reporter dyes compatible with common instrumentation. There is an increase of signal at 670 nm when TdT is present. 0000024431 00000 n 519. xref 0000004885 00000 n 0000001090 00000 n 0000001284 00000 n 0000009429 00000 n TAMRA Absorbance. 0000002294 00000 n 0000001616 00000 n The dye has an absorption wavelength that peaks around 495 nm, and an emission maximum around 517 nm. 528. We have developed the new 0000011458 00000 n 0000002106 00000 n TAMRA Emission. Below pH 7, 6-FAMâ¢ becomes protonated, which results in decreased fluorescence. Choose dyes with appropriate excitation wavelengths and little to no overlap in their emission spectra. 0000004754 00000 n 5' TAMRA-T10 oligonucleotide in PCR buffer . When using multiple dyes (multiplex) the excitation and emission wavelengths of each dye must be independent to avoid cross talk; Quenchers Quenching molecules are typically placed at the 3' end of single molecule probes such as Dual-Labeled Probes, Molecular Beacons and Scorpions Probes. Fluorescein is an organic compound and dye. 76 43 The donor dye (fluorescein) is excited by the blue LED. Consider total ï¬uorescence intensity as well. This structure features a linker between Coumarin 343 fluorophore, and the functional group. NBD-X. Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). Spectral Properties . %PDF-1.4 %�� 0000001156 00000 n Fluorophores are very useful and sensitive labels for biomolecules, and fluorescent labels are by far the most common labels used for synthetic oligonucleotides. Emission spectra for FAM, VIC, ABY, and JUN dyes are shown in relation to regions of the spectrum detected by six filters available on Applied Biosystems real-time PCR instruments. MP = Mustang Purpleâ¢ dye. 0000042457 00000 n The following data tables provide you with information on the fluorescent lifetimes, excitation and emission wavelengths of Selected fluorescent dyes, probes and labels that are frequently used for biological applications and in biomedical research. LGC Biosearch Technologies' series of Quasar® dyes are direct, cost-effective replacements for the Cyâ¢3, Cy5 and Cy5.5 dyes. 0000003066 00000 n 0000069316 00000 n The absorption spectrum of a compound thus is the ï¬rst expression of possible photoactivity. 0000024631 00000 n wavelength of absorption and the quantitative issue of the inten-sity of absorption. 0000030517 00000 n H��V�v�0��+��v�=�Rާ��9�E��@��r�� 0000008309 00000 n four reporter dyes, a new quencher, and a new master mix. TaqMan MGB probes (Figure 8) comprise a reporter dye (6-FAM) linked to the 5â end of the probe, and an NFQ at the 3â end. 0000001404 00000 n 0000078488 00000 n ��O�22^&�FXn�*Ne��N��7��ɛ� �çh�?.��>#� �!�s�#ڨ�RL1_�HQ�y��LXR\'��*I��gǧ=R��ǯz��E�+0��S(R��k2��9�&��O^&��$Z��s��9�n�N��;� �}�N��e�g=g�٨$�&i&�7��j>�E��}��Y]�Ѽ�/�jA~'A [�6�_z��|1��7�Th:�_M��j�50 *��4�VH�r�*��,��'΅��*��K�m�2��|�Ea@��j��#�f-��!��tX��fH.�+�4�IN��T��v��JN��|Kj'$��/�g��y�qT��5E�Kem��t�Ƹ��EY��'z>\��OiȐmR�G��ѻ@_{py@[*p�x�t�``�Yɨ��i�(dj[2a��bA�j. Select the appropriate probe format according to sample type and application by visiting our Probe Application Chart and clicking on the links to go to the product page for the corresponding probe to learn more. 495. The energy transfer occurs in one of two ways, depending on the chemical structure of the acceptor if the donor and acceptor dyes are in close proximity (10â100Å): It provides better solubility, and spatial separation between the dye, and the functional group. Wavelength (nm) FAM Absorption and Emission Spectra . NFQ is a proprietary molecule that is effective over a broad wavelength range (FAMâ¢, VICâ¢ and TETâ¢ dyes, www.appliedbiosystems.com). As long as the two dyes are close to each other (within 15 nucleotides), the energy emitted by the donor dye excites the acceptor dye on the second HybProbe probe, which then emits fluorescent light at a different wavelength. This can be accomplished using non-specific DNA binding dyes or sequence-specific probes. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength. Wavelength scan from 480 nm to 700 nm with excitation at 450 nm. 0000017605 00000 n I think one of the most common fluorophores for the Taqman probe is FAM with an emission wavelength 520 nm and TET with 536 nm. 0000010590 00000 n 76 0 obj <> endobj 466. 520. ( Figure 2 ) similarly to a chromophore ) is excited at a single dye excited... 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